Category: Mitosis

Cells were centrifuged at 1000 rpm for 5 min, and excess media were aspirated

Cells were centrifuged at 1000 rpm for 5 min, and excess media were aspirated. TUGUL modifies the kinesin motor protein, KIF5B, and that TUG proteolysis is required to load GLUT4 onto these motors. Insulin stimulates TUG proteolytic processing independently PMPA of phosphatidylinositol 3-kinase. In nonadipocytes, TUG cleavage can be reconstituted by transfection of Usp25m, but […]